Single-cell analyses to reveal hematopoietic stem cell fate decisions

被引：9

作者：

Lunger, Ilaria ^{[1
]}

Fawaz, Malak ^{[1
]}

Rieger, Michael A. ^{[1
,2
,3
]}

机构：

[1] Goethe Univ Frankfurt, Dept Med Hematol Oncol, Theodor Stern Kai 7, D-60590 Frankfurt, Germany

[2] German Canc Consortium DKTK, Heidelberg, Germany

[3] German Canc Res Ctr, Heidelberg, Germany

来源：

FEBS LETTERS | 2017年 / 591卷 / 15期

关键词：

molecular and functional single-cell technologies; omics; sequencing and lineage tracing; single cell tracking; time-lapse microscopy; blood reconstitution; ACUTE LYMPHOBLASTIC-LEUKEMIA; COLONY-STIMULATING FACTOR; RNA-SEQUENCING REVEALS; MOUSE BONE-MARROW; IN-VIVO; SELF-RENEWAL; LINEAGE COMMITMENT; CLONAL ANALYSIS; MASS CYTOMETRY; EPIGENETIC HETEROGENEITY;

D O I：

10.1002/1873-3468.12712

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Hematopoietic stem cells (HSCs) are the best studied adult stem cells with enormous clinical value. Most of our knowledge about their biology relies on assays at the single HSC level. However, only the recent advances in developing new single cell technologies allowed the elucidation of the complex regulation of HSC fate decision control. This Review will focus on current attempts to investigate individual HSCs at molecular and functional levels. The advantages of these technologies leading to groundbreaking insights into hematopoiesis will be highlighted, and the challenges facing these technologies will be discussed. The importance of combining molecular and functional assays to enlighten regulatory networks of HSC fate decision control, ideally at high temporal resolution, becomes apparent for future studies.

引用

页码：2195 / 2212

页数：18

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