Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity

被引：496

作者：

Angermueller, Christof ^{[1
]}

Clark, Stephen J. ^{[2
]}

Lee, Heather J. ^{[2
,3
]}

Macaulay, Iain C. ^{[3
]}

Teng, Mabel J. ^{[3
]}

Hu, Tim Xiaoming ^{[1
,3
,4
]}

Krueger, Felix ^{[5
]}

Smallwood, Sebastien A. ^{[2
]}

Ponting, Chris P. ^{[3
,4
]}

Voet, Thierry ^{[3
,6
]}

Kelsey, Gavin ^{[2
]}

Stegle, Oliver ^{[1
]}

Reik, Wolf ^{[2
,3
]}

机构：

[1] European Bioinformat Inst, European Mol Biol Lab, Cambridge, England

[2] Babraham Inst, Epigenet Programme, Cambridge, England

[3] Wellcome Trust Sanger Inst, Cambridge, England

[4] Univ Oxford, MRC, Funct Genom Unit, Oxford, England

[5] Babraham Inst, Bioinformat Grp, Cambridge, England

[6] Katholieke Univ Leuven, Dept Human Genet, Leuven, Belgium

来源：

NATURE METHODS | 2016年 / 13卷 / 03期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会; 英国医学研究理事会;

关键词：

DNA METHYLATION; STEM-CELLS; METHYLOME; GENOME; PLURIPOTENCY; EMBRYOS; NANOG; STATE;

D O I：

10.1038/nmeth.3728

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.

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收藏

页码：229 / +

页数：6

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