Characterization of the BcLF1 promoter in Epstein-Barr virus

被引：8

作者：

Chang, PJ

Chang, YS

Liu, ST ^{[1
]}

机构：

[1] Chang Gung Univ, Dept Microbiol & Immunol, Mol Genet Lab, Tao Yuan 333, Taiwan

[2] Natl Yang Ming Univ, Inst Microbiol & Immunol, Taipei 112, Taiwan

来源：

JOURNAL OF GENERAL VIROLOGY | 1998年 / 79卷

关键词：

D O I：

10.1099/0022-1317-79-8-2003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The location of the BcLF1 promoter of Epstein-Barr virus (EBV) has been identified by primer extension, which indicates that the +1 site of the BcLF1 mRNA is located at nucleotide 137676 of the EBV genome. According to deletion analysis, the region upstream from nucleotide -38 is not essential for transcription of BcLF1. A 23 bp region in the promoter, from nucleotide -38 to -16, was identified as necessary for regulating the expression of BcLF1, i.e. the promoter activity is activated by 12-O-tetradecanoylphorbol 13-acetate but is repressed by phosphonoacetic acid. The results presented also demonstrate that the oriLyt sequence in cis is essential for enhancing the expression of BcLF1.

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页码：2003 / 2006

页数：4

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