Protein changes in response to progressive water deficit in maize - Quantitative variation and polypeptide identification

被引：308

作者：

Riccardi, F ^{[1
]}

Gazeau, P ^{[1
]}

de Vienne, D ^{[1
]}

Zivy, M ^{[1
]}

机构：

[1] Univ Paris Sud, Inst Natl Rech Agron, Inst Natl Agron Paris Grignon, CNRS,URA 2154,Stn Genet Vegetale, F-91190 Gif Sur Yvette, France

来源：

PLANT PHYSIOLOGY | 1998年 / 117卷 / 04期

关键词：

D O I：

10.1104/pp.117.4.1253

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Three-week-old plants of two unrelated lines of maize (ilea mays L.) and their hybrid were submitted to progressive water stress for 10 d. Changes induced in leaf proteins were studied by two-dimensional electrophoresis and quantitatively analyzed using image analysis. Seventy-eight proteins out of a total of 413 showed a significant quantitative variation (increase or decrease), with 38 of them exhibiting a different expression in the two genotypes. Eleven proteins that increased by a factor of 1.3 to 5 in stressed plants and 8 proteins detected only in stressed plants were selected for internal amino acid microsequencing, and by similarity search 16 were found to be closely related to previously reported proteins. In addition to proteins already known to be involved in the response to water stress (e.g. RAB17 [Responsive to ABA]), Several enzymes involved in basic metabolic cellular pathways such as glycolysis and the Krebs cycle (e.g. enolase and triose phosphate isomerase) were identified, as well as several others, including caffeate O-methyltransferase, the induction of which could be related to lignification.

引用

页码：1253 / 1263

页数：11

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