Optimization of free radical scavenging activity by response surface methodology in the hydrolysis of shrimp processing discards

被引：120

作者：

Guerard, Fabienne ^{[1
]}

Sumaya-Martinez, Maria Teresa

Laroque, Delphine

Chabeaud, Aurehe

Dufosse, Laurent

机构：

[1] Univ Bretagne Occidentale, Lab ANTiOX, Pole Univ PJ Helias, F-29000 Creach Gwen, Quimper, France

[2] Univ La Reunion, Lab Chim Subst Nat & Sci Aliments, F-97715 St Denis 9, Reunion, France

来源：

PROCESS BIOCHEMISTRY | 2007年 / 42卷 / 11期

关键词：

Alcalase; (R); 2; AL; antioxidant activity; shrimp by-product; hydrolysis; response surface methodology (RSM);

D O I：

10.1016/j.procbio.2007.07.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Response surface methodology (RSM) was used to optimize the hydrolysis conditions (temperature, pH and Alcalase (R) 2,4L concentration), in order to obtain the hydrolysate with the strongest antioxidant activity using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH.) decolouration assay. The optimum conditions obtained from experiments were pH 9.7, 66.2 degrees C, enzyme concentration = 68.1 Anson units (AU)/kg crude protein. The analysis of variance in RSM showed that pH and temperature (T) were the most important factors of the process (P < 0.001). The experimental conditions produced both an enzymatic and chemical protein solubilization. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1486 / 1491

页数：6

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