Augmentation of macrophage functions by an aqueous extract isolated from Platycodon grandiflorum

被引:94
作者
Choi, CY
Kim, JY
Kim, YS
Chung, YC
Hahm, KS
Jeong, HG
机构
[1] Chosun Univ, Dept Pharm, Kwangju 501759, South Korea
[2] Chosun Univ, Res Ctr Proteineous Mat, Kwangju 501759, South Korea
[3] Korea Res Inst Chem Technol, Taejon 305606, South Korea
[4] Coll Chinju, Dept Food Nutr, Chinju, South Korea
关键词
Platycodon grandiflorum; macrophages; activation; cytokine;
D O I
10.1016/S0304-3835(01)00440-2
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Platycodon grandiflorum has been claimed to have a wide range of health benefits, which include immunostimulation and antitumor activity. The associated biological mechanisms are unclear; however, of the wide diversity of effects, it is believed that their activities may be exerted through several potent effector cells such as macrophages. Therefore, the effects of an aqueous extract from the root of P. grandiflorum (Changkil: CK) on mouse peritoneal macrophage function were investigated. It was found that CK stimulated macrophage proliferation. spreading ability, phagocytosis, cytostatic activity, and nitric oxide production in a dose-dependent manner, and that the production of cytokines such as TNF-alpha, IL-1 beta and IL-6 were similarly increased. CK significantly affected secretion at concentrations greater than 10 mug/ml; its maximal effects were at the concentration of 100 mug/ml. Reverse transcription-polymerase chain reaction showed that CK increased the appropriate cytokine mRNAs. These results suggest that CK is a potent enhancer of macrophage function. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:17 / 25
页数:9
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