Bacteriorhodopsin/amphipol complexes: Structural and functional properties

被引：79

作者：

Gohon, Yann ^{[3
,4
]}

Dahmane, Tassadite ^{[3
,4
]}

Ruigrok, Rob W. H. ^{[5
]}

Schuck, Peter

Charvolin, Delphine

Rappaport, Fabrice ^{[7
,8
]}

Timmins, Peter ^{[9
]}

Engelman, Donald M. ^{[10
]}

Tribet, Christophe ^{[11
]}

Popot, Jean-Luc ^{[3
,4
]}

Ebel, Christine ^{[1
,2
,6
]}

机构：

[1] Univ Grenoble 1, F-38027 Grenoble, France

[2] IBS, CNRS, Mol Biophys Lab, CEA,DSV, F-38027 Grenoble, France

[3] Univ Paris 07, Inst Biol Phys Chim, F-75005 Paris, France

[4] CNRS, Lab Phys Chim Mol Membranes Biol, F-75005 Paris, France

[5] Unit Virus Host Cell Interact, Grenoble, France

[6] NIH, PBR, DBPS, ORS, Bethesda, MD USA

[7] CNRS, Paris, France

[8] Univ Paris 06, Inst Biol Phys Chim, Paris, France

[9] Inst Max Von Laue Paul Langevin, Large Scale Struct Grp, Grenoble, France

[10] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

[11] CNRS, Lab Phys Chim Polymeres & Milieux Disperses, Paris, France

来源：

BIOPHYSICAL JOURNAL | 2008年 / 94卷 / 09期

关键词：

D O I：

10.1529/biophysj.107.121848

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The membrane protein bacteriorhodopsin ( BR) can be kept soluble in its native state for months in the absence of detergent by amphipol (APol) A8-35, an amphiphilic polymer. After an actinic. ash, A8-35-complexed BR undergoes a complete photocycle, with kinetics intermediate between that in detergent solution and that in its native membrane. BR/APol complexes form well defined, globular particles comprising a monomer of BR, a complete set of purple membrane lipids, and, in a peripheral distribution, similar to 2 g APol/g BR, arranged in a compact layer. In the absence of free APol, BR/APol particles can autoassociate into small or large ordered fibrils.

引用

页码：3523 / 3537

页数：15

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