Simultaneous determination of 5-fluorouracil and uracil by high-performance liquid chromatography using four serial columns

被引：22

作者：

House, LK

Ramírez, J

Ratain, MJ

机构：

[1] Univ Chicago, Dept Med, Hematol Oncol Sect, Chicago, IL 60637 USA

[2] Univ Chicago, Comm Clin Pharmacol, Chicago, IL 60637 USA

[3] Univ Chicago, Ctr Canc Res, Chicago, IL 60637 USA

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 720卷 / 1-2期

关键词：

uracil; 5-fluorouracil;

D O I：

10.1016/S0378-4347(98)00424-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive assay was developed for the quantitation of 5-fluorouracil (5-FU) and uracil using liquid-liquid extraction (LLE) and HPLC with UV detection. Analyses were performed with four mu Bondapak C-18 columns connected in series using 20 mM acetic acid with 1% ACN as mobile phase. The calibration curves were linear across the range of 26-1000 ng ml(-1) (0.21-7.8 mu M) for 5-FU and 1.0-14.0 mu g ml(-1) (0.01-110 mu M) for uracil. This assay has been implemented to determine the plasma concentrations for pharmacokinetic studies for 5-FU and uracil in conjunction with clinical trials. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：245 / 250

页数：6