Interaction of human cyclophilin hCyp-18 with short peptides suggests the existence of two functionally independent subsites

被引：16

作者：

Demange, L ^{[1
]}

Moutiez, M ^{[1
]}

Vaudry, K ^{[1
]}

Dugave, C ^{[1
]}

机构：

[1] CEA Saclay, Dept Ingn & Etud Prot, F-91191 Gif Sur Yvette, France

来源：

FEBS LETTERS | 2001年 / 505卷 / 01期

关键词：

peptidyl-prolyl cis/trans isomerase; proline; cis/trans isomerization; subsite;

D O I：

10.1016/S0014-5793(01)02814-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The binding of peptides, derived from the model substrate Suc-Ala-Ala-Pro-Phe-pNA, to the human cyclophilin hCyp-18 was investigated. HCyp-18 is able to bind 2-4-mer peptides as well as shorter para-nitroaniline (pNA) derivatives and pNA surrogates. Although Suc-Ala-Phe-pNA binds hCyp-18, only proline-containing peptides are able to block efficiently the peptidyl-prolyl cis/trans isomerase activity. Competition experiments strongly suggest the existence of two independent subsites: a S1 ' 'Proline' subsite and a S2 ' -S3 ' 'pNA' subsite. The interaction at S2 ' -S3 ' requires either a Phe-pNA C-terminus or a Phe-pNA surrogate bearing an H-bond acceptor able to bind Trp121 and Arg148 simultaneously. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：191 / 195

页数：5

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