Three-dimensional in vitro model of adipogenesis:: Comparison of culture conditions

被引:47
作者
Fischbach, C
Seufert, J
Staiger, H
Hacker, M
Neubauer, M
Göpferich, A
Blunk, T [1 ]
机构
[1] Univ Regensburg, Dept Pharmaceut Technol, D-93040 Regensburg, Germany
[2] Univ Wurzburg, Med Poliklin, Div Endocrinol Metab & Mol Med, D-8700 Wurzburg, Germany
[3] Univ Tubingen, Med Clin, Dept Endocrinol Metab & Pathobiochem, D-7400 Tubingen, Germany
来源
TISSUE ENGINEERING | 2004年 / 10卷 / 1-2期
关键词
D O I
10.1089/107632704322791862
中图分类号
Q813 [细胞工程];
学科分类号
摘要
In vivo and in vitro studies have demonstrated both promise and current limitations in tissue engineering of fat. Herein, we report the establishment of a well-defined three-dimensional (3-D) in vitro model useful for systematic investigations of 3-D adipogenesis. Polyglycolic acid fiber meshes were dynamically seeded with 3T3-L1 preadipocytes; subsequently, cell-polymer constructs were hormonally induced and cultivation under three different conditions was evaluated. Regarding tissue coherence and intracellular lipid content, culture of cell-polymer constructs either dynamically in well plates or in stirred bioreactors yielded similar results, which were distinctly improved compared with static conditions in well plates. At the protein and mRNA levels, significantly increased expression of genes characteristic for a mature adipose phenotype was demonstrated for constructs dynamically cultured in well plates, as compared with static conditions. Furthermore, investigation of lipolysis under stimulating and inhibiting conditions demonstrated functionality of the dynamically differentiated constructs. Using dynamic culture conditions, the presented in vitro model system is suggested as a valuable tool serving both fat tissue engineering and basic research by facilitating investigations of tissue-inherent features not possible under conventional 2-D culture conditions.
引用
收藏
页码:215 / 229
页数:15
相关论文
共 52 条
[41]   FIBRONECTIN MODULATION OF CELL-SHAPE AND LIPOGENIC GENE-EXPRESSION IN 3T3-ADIPOCYTES [J].
SPIEGELMAN, BM ;
GINTY, CA .
CELL, 1983, 35 (03) :657-666
[42]   DECREASES IN TUBULIN AND ACTIN GENE-EXPRESSION PRIOR TO MORPHOLOGICAL-DIFFERENTIATION OF 3T3 ADIPOCYTES [J].
SPIEGELMAN, BM ;
FARMER, SR .
CELL, 1982, 29 (01) :53-60
[43]  
SUGIHARA H, 1987, J LIPID RES, V28, P1038
[44]   De novo formation of adipose tissue by controlled release of basic fibroblast growth factor [J].
Tabata, Y ;
Miyao, M ;
Inamoto, T ;
Ishii, T ;
Hirano, Y ;
Yamaoki, Y ;
Ikada, Y .
TISSUE ENGINEERING, 2000, 6 (03) :279-289
[45]   Endogenous adipocyte precursor cells for regenerative soft-tissue engineering [J].
Toriyama, K ;
Kawaguchi, N ;
Kitoh, J ;
Tajima, R ;
Inou, K ;
Kitagawa, Y ;
Torii, S .
TISSUE ENGINEERING, 2002, 8 (01) :157-165
[46]   Human preadipocytes seeded on freeze-dried collagen scaffolds investigated in vitro and in vivo [J].
von Heimburg, D ;
Zachariah, S ;
Heschel, I ;
Kühling, H ;
Schoof, H ;
Hafemann, B ;
Pallua, N .
BIOMATERIALS, 2001, 22 (05) :429-438
[47]   Influence of different biodegradable carriers on the in vivo behavior of human adipose precursor cells [J].
von Heimburg, D ;
Zachariah, S ;
Low, A ;
Pallua, N .
PLASTIC AND RECONSTRUCTIVE SURGERY, 2001, 108 (02) :411-420
[48]   Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering [J].
Vunjak-Novakovic, G ;
Obradovic, B ;
Martin, I ;
Bursac, PM ;
Langer, R ;
Freed, LE .
BIOTECHNOLOGY PROGRESS, 1998, 14 (02) :193-202
[49]   Effects of mixing on the composition and morphology of tissue-engineered cartilage [J].
VunjakNovakovic, G ;
Freed, LE ;
Biron, RJ ;
Langer, R .
AICHE JOURNAL, 1996, 42 (03) :850-860
[50]   Augmentation of adipofascial flaps using the long-term local delivery of insulin and insulin-like growth factor-1 [J].
Yuksel, E ;
Weinfeld, AB ;
Cleek, R ;
Jensen, J ;
Wamsley, S ;
Waugh, JM ;
Spira, M ;
Shenaq, S .
PLASTIC AND RECONSTRUCTIVE SURGERY, 2000, 106 (02) :373-382