Titanium nitride oxide coating on rough titanium stimulates the proliferation of human primary osteoblasts

Objectives Titanium is widely used in contemporary endosseous implantology and there is considerable thrust to further promote osseointegration by implant surface modifications. The aim of this study was to evaluate the effect of a titanium-nitride-oxide (TiNOx) coating on commercially pure microroughened titanium by assessing the proliferation and differentiation of human primary osteoblasts. Materials and methods Cell proliferation, gene expression, alkaline phosphatase activity, osteoprotegerin and osteocalcin secretion were analyzed for a time course of 3 weeks, with or without additional stimulation by 1.25(OH)(2) vitamin D-3 100 nM. Results A 1.5-fold increase in the proliferation rate of cells grown on TiNOx-coated titanium as compared with uncoated surfaces was observed. SEM views indicated that the cells' normal morphology with their numerous extensions was maintained. The differentiation process on the TiNOx surface was only affected to a minor degree and translated into a slight delay in osteoblast maturation when compared to uncoated titanium. Conclusion Pending confirmation of these results in vivo, TiNOx coatings could potentially accelerate and enhance osseointegration. To cite this article:Durual S, Pernet F, Rieder P, Mekki M, Cattani-Lorente M, Wiskott H. W. A. Titanium nitride oxide coating on rough titanium stimulates the proliferation of human primary osteoblasts.Clin. Oral Impl. Res. 22, 2011; 552-559doi: 10.1111/j.1600-0501.2010.02033.x.