An insulin-like peptide regulates egg maturation and metabolism in the mosquito Aedes aegypti

被引：196

作者：

Brown, Mark R. ^{[1
]}

Clark, Kevin D. ^{[1
]}

Gulia, Monika ^{[1
]}

Zhao, Zhangwu ^{[1
]}

Garczynski, Stephen F. ^{[1
]}

Crim, Joe W. ^{[2
]}

Sulderman, Richard J. ^{[1
]}

Strand, Michael R. ^{[1
]}

机构：

[1] Univ Georgia, Dept Entomol, Athens, GA 30602 USA

[2] Univ Georgia, Dept Cell Biol, Athens, GA 30602 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 15期

关键词：

endocrinology; insect; reproduction; vector;

D O I：

10.1073/pnas.0800478105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ingestion of vertebrate blood is essential for egg maturation and transmission of disease-causing parasites by female mosquitoes. Prior studies with the yellow fever mosquito, Aedes aegypti, indicated blood feeding stimulates egg production by triggering the release of hormones from medial neurosecretory cells in the mosquito brain. The ability of bovine insulin to stimulate a similar response further suggested this trigger is an endogenous insulin-like peptide (ILP). A. aegypti encodes eight predicted ILPs. Here, we report that synthetic ILP3 dose-dependently stimulated yolk uptake by oocytes and ecdysteroid production by the ovaries at lower concentrations than bovine insulin. ILP3 also exhibited metabolic activity by elevating carbohydrate and lipid storage. Binding studies using ovary membranes indicated that ILP3 had an IC50 value of 5.9 nM that was poorly competed by bovine insulin. Autoradiography and immunoblotting studies suggested that ILP3 binds the mosquito insulin receptor (MIR), whereas loss-of-function experiments showed that ILP3 activity requires MIR expression. Overall, our results identify ILP3 as a critical regulator of egg production by A. aegypti.

引用

页码：5716 / 5721

页数：6

共 50 条

[1] Nutritional regulation of vitellogenesis in mosquitoes: Implications for anautogeny [J].