Directed evolution of enzymes for biocatalytic applications

被引：16

作者：

Bornscheuer, UT ^{[1
]}

机构：

[1] Ernst Moritz Arndt Univ Greifswald, Dept Tech Chem & Biotechnol, Inst Chem & Biochem, D-17487 Greifswald, Germany

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2001年 / 19卷 / 02期

关键词：

directed evolution; DNA-shuffling; enzymes; error-prone PCR; high-throughput screening; molecular evolution;

D O I：

10.3109/10242420109003638

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Directed molecular evolution is a rapidly growing field revolutionizing the development of biocatalysts with improved properties. This review describes methods to create mutant libraries and assays for rapid screening or selection of desired variants. Selected examples emphasizing the evolution of enzymes for applications in biocatalysis show that it is possible to alter substrate specificity, modulate enantio selectivity and increase enzyme performance under process conditions.

引用

页码：85 / 97

页数：13

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