Identification of genes involved in cytochrome c biogenesis in Shewanella oneidensis, using a modified mariner transposon

被引：80

作者：

Bouhenni, R ^{[1
]}

Gehrke, A ^{[1
]}

Saffarini, D ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53211 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 08期

关键词：

D O I：

10.1128/AEM.71.8.4935-4937.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon insertions in the cytochrome c maturation genes ccmC and ccmF1, respectively, were analyzed. Both mutants were deficient in anaerobic respiration and cytochrome c production.

引用

页码：4935 / 4937

页数：3

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