New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples

被引：49

作者：

Casson, Nicola ^{[1
,2
]}

Posfay-Barbe, Klara M. ^{[3
]}

Gervaix, Alain ^{[3
]}

Greub, Gilbert ^{[1
,2
]}

机构：

[1] Univ Hosp Ctr, Ctr Res Intracellular Bacteria, Inst Microbiol, CH-1011 Lausanne, Switzerland

[2] Univ Lausanne, CH-1011 Lausanne, Switzerland

[3] Univ Hosp Geneva, Childrens Hosp Geneva, Geneva, Switzerland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 04期

关键词：

D O I：

10.1128/JCM.02302-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia. The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.

引用

页码：1491 / 1493

页数：3

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