The growth inhibitory effect of mesenchymal stem cells on tumor cells in vitro and in vivo

被引：173

作者：

Lu, Yan-rong ^{[1
]}

Yuan, Yu ^{[1
]}

Wang, Xiu-jie ^{[2
]}

Wei, Ling-ling ^{[1
]}

Chen, You-nan ^{[1
]}

Cong Cong ^{[1
]}

Li, Sheng-fu ^{[1
]}

Long, Dan ^{[1
]}

Tan, Wei-dong ^{[1
]}

Mao, Ying-qiu ^{[2
]}

Zhang, Jie ^{[1
]}

Li, You-ping ^{[1
]}

Cheng, Jing-qiu ^{[1
]}

机构：

[1] Sichuan Univ, W China Hosp, Key Lab Transplant Engn & Immunol, Minist Hlth, Chengdu 610041, Peoples R China

[2] Sichuan Univ, W China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China

来源：

CANCER BIOLOGY & THERAPY | 2008年 / 7卷 / 02期

基金：

中国国家自然科学基金;

关键词：

mesenchymal stem cells (MSCs); tumor; growth inhibition; apoptosis;

D O I：

10.4161/cbt.7.2.5296

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Mesenchymal stem cells (MSCs) play an important role in the development and growth of tumor cells. The purpose of this study is to confirm the effect of MSCs on tumor cell growth in vitro and in vivo and to elucidate the mechanism. MSCs were isolated from mouse bone marrow and cocultured with murine hepatoma H22, lymphoma (YAC-1 and EL-4) and rat insulinoma INS-1 cell lines. The growth inhibitory effect of MSCs on tumor cells was tested through MTT and 3H-TdR incorporation assay. The apoptosis induction effect of MSCs on tumor cells was assessed with flow cytometry (FCM) and RT-PCR assay. MSCs were inoculated into BALB/c mice alone or coinoculated with ascitogenous hepatoma cells intraperitonealy, respectively. The tumor growth inhibition of MSCs was investigaed through the incidence and volume of ascites formation, and the immunosuppression effect was studied with splenocyte response to ConA stimulation test and T cell subsets analysis (FCM). The results showed that MSCs exhibited a number-dependent growth inhibitory effect on murine tumor cell lines in vitro and inhibited the growth of ascitogenous hepatoma cells in vivo without host immunosuppression. MSCs could upregulate tumor cells mRNA expression of cell cycle negative regulator p21 and apoptosis associated protease caspase 3. The findings of this experimental study demonstrated that MSCs had potential inhibitory effects on tumor cell growth in vitro and in vivo without host immunosuppression, by inducing apoptotic cell death and G(0)/G(1) phase arrest of cancer cells.

引用

页码：245 / 251

页数：7

共 21 条

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