A simple and specific assay for real-time colorimetric visualization of β-lactamase activity by using gold nanoparticles

被引：92

作者：

Liu, Rongrong ^{[1
]}

Liew, Roushen ^{[1
]}

Zhou, He ^{[1
]}

Xing, Bengang ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Math & Phys Sci, Div Chem & Biol Chem, Singapore 637616, Singapore

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 46期

关键词：

beta-lactamase; bacterial cells; colorimetric assays; enzymes; nanoparticles;

D O I：

10.1002/anie.200702773

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) The color of gold : Bacterial β-lactamases (Blas) can be rapidly identified by a simple and specific colorimetric assay with gold nanoparticles (Au-NPs). This assay can evaluate enzymatic kinetic reactions and screen inhibitors of Blas in real time, and offers a simple and economical method for the rapid detection of β-lactam resistant bacteria in clinical studies. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：8799 / 8803

页数：5

共 38 条

[1]

[Anonymous], 2007, ANGEW CHEM INT ED, DOI DOI 10.1002/ANGE.200605249

[2] Nanogold-plasmon-resonance-based glucose sensing [J].

Aslan, K ;

Lakowicz, JR ;

Geddes, CD .

ANALYTICAL BIOCHEMISTRY, 2004, 330 (01) :145-155

[3] A FUNCTIONAL CLASSIFICATION SCHEME FOR BETA-LACTAMASES AND ITS CORRELATION WITH MOLECULAR-STRUCTURE [J].

BUSH, K ;

JACOBY, GA ;

MEDEIROS, AA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (06) :1211-1233

[4]

CHOI Y, 2007, ANGEW CHEM, V119, P721

[5] Sensing phosphatase activity by using gold nanoparticles [J].

Choi, Yongdoo ;

Ho, Nan-Hui ;

Tung, Ching-Hsuan .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (05) :707-709

[6]

de la Fuente J.M., 2001, ANGEW CHEM, V773, P2317, DOI DOI 10.1021/ac062341m

[7]

de la Fuente JM, 2001, ANGEW CHEM INT EDIT, V40, P2258, DOI 10.1002/1521-3773(20010618)40:12<2257::AID-ANIE2257>3.0.CO

[8]

2-S

[9] Gold nanoparticles as spectroscopic enhancers for in vitro studies on single viruses [J].

Dragnea, B ;

Chen, C ;

Kwak, ES ;

Stein, B ;

Kao, CC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (21) :6374-6375

[10] Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].

Elghanian, R ;

Storhoff, JJ ;

Mucic, RC ;

Letsinger, RL ;

Mirkin, CA .

SCIENCE, 1997, 277 (5329) :1078-1081

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