Molecular mechanisms of kinetochore capture by spindle microtubules

被引:221
作者
Tanaka, K [1 ]
Mukae, N
Dewar, H
van Breugel, M
James, EK
Prescott, AR
Antony, C
Tanaka, TU
机构
[1] Univ Dundee, Sch Life Sci, Wellcome Trust Bioctr, Dundee DD1 5EH, Scotland
[2] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany
[3] European Mol Biol Lab, D-69117 Heidelberg, Germany
基金
英国惠康基金;
关键词
D O I
10.1038/nature03483
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
For high-fidelity chromosome segregation, kinetochores must be properly captured by spindle microtubules, but the mechanisms underlying initial kinetochore capture have remained elusive. Here we visualized individual kinetochore-microtubule interactions in Saccharomyces cerevisiae by regulating the activity of a centromere. Kinetochores are captured by the side of microtubules extending from spindle poles, and are subsequently transported poleward along them. The microtubule extension from spindle poles requires microtubule plus-end-tracking proteins and the Ran GDP/GTP exchange factor. Distinct kinetochore components are used for kinetochore capture by microtubules and for ensuring subsequent sister kinetochore bi-orientation on the spindle. Kar3, a kinesin-14 family member, is one of the regulators that promote transport of captured kinetochores along microtubules. During such transport, kinetochores ensure that they do not slide off their associated microtubules by facilitating the conversion of microtubule dynamics from shrinkage to growth at the plus ends. This conversion is promoted by the transport of Stu2 from the captured kinetochores to the plus ends of microtubules.
引用
收藏
页码:987 / 994
页数:8
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