Use of the indirect competitive ELISA for the detection of Brazil nut in food products

被引：25

作者：

Clemente, A ^{[1
]}

Chambers, SJ ^{[1
]}

Lodi, F ^{[1
]}

Nicoletti, C ^{[1
]}

Brett, GM ^{[1
]}

机构：

[1] Inst Food Res, Nutr & Consumer Sci Div, Norwich NR4 7UA, Norfolk, England

来源：

FOOD CONTROL | 2004年 / 15卷 / 01期

关键词：

allergy; Brazil nut; immunoassay;

D O I：

10.1016/S0956-7135(03)00017-3

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Food related allergic reactions following inadvertent ingestion are increasingly common. with nuts, including Brazil nut, placed firmly in the top 10 food groups whose presence within a product should be declared. The presence of hidden allergens as a result Of adulteration or contamination of ingredients presents a problem for both the food industry and the consumer. A sensitive and specific immunoassay for Brazil nut is described with a limit of detection of 1 ppm. Based upon the detection of the abundant 2S protein the assay is suitable for detection of raw and roasted Brazil nut ill a range of food matrices. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：65 / 69

页数：5

共 23 条

[1] Five cases of food allergy to Brazil nuts in children [J].

Bourrier, T ;

Villevieille, L ;

Albertini, M ;

Boutte, P .

REVUE FRANCAISE D ALLERGOLOGIE ET D IMMUNOLOGIE CLINIQUE, 2001, 41 (04) :401-406

[2] Design and development of immunoassays for detection of proteins [J].

Brett, GM ;

Chambers, SJ ;

Huang, L ;

Morgan, MRA .

FOOD CONTROL, 1999, 10 (06) :401-406

[3]

Dutau G, 1999, PRESSE MED, V28, P1553

[4]

ERIKSSON A, 1992, FOOD SAFETY QUALITY

[5] A SANDWICH ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR THE QUANTITATION OF SELECTED PEANUT PROTEINS IN FOODS [J].

HEFLE, SL ;

BUSH, RK ;

YUNGINGER, JW ;

CHU, FS .

JOURNAL OF FOOD PROTECTION, 1994, 57 (05) :419-423

[6] A sandwich enzyme-linked immunosorbent assay for the detection of almonds in foods [J].

Hlywka, JJ ;

Hefle, SL ;

Taylor, SL .

JOURNAL OF FOOD PROTECTION, 2000, 63 (02) :252-257

[7] Quantitative sandwich ELISA for determination of traces of hazelnut (Corylus avellana) protein in complex food matrixes [J].

Holzhauser, T ;

Vieths, S .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (10) :4209-4218

[8] Prevalence and severity of food allergy need for control [J].

Hourihane, JO .

ALLERGY, 1998, 53 :84-88

[9]

Hourihane JO, 1997, CLIN EXP ALLERGY, V27, P1240, DOI 10.1046/j.1365-2222.1997.1520954.x

[10] An evaluation of the sensitivity of subjects with peanut allergy to very low doses of peanut protein: A randomized, double-blind, placebo-controlled food challenge study [J].

Hourihane, JO ;

Kilburn, SA ;

Nordlee, JA ;

Hefle, SL ;

Taylor, SL ;

Warner, JO .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1997, 100 (05) :596-600

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