Design and development of immunoassays for detection of proteins

被引：55

作者：

Brett, GM ^{[1
]}

Chambers, SJ ^{[1
]}

Huang, L ^{[1
]}

Morgan, MRA ^{[1
]}

机构：

[1] Food Res Inst, Dept Biochem, Norwich NR4 7UA, Norfolk, England

来源：

FOOD CONTROL | 1999年 / 10卷 / 06期

基金：

英国生物技术与生命科学研究理事会;

关键词：

immunoassay development; antibodies; GMO detection;

D O I：

10.1016/S0956-7135(99)00082-1

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Immunoassay methodology is currently the method of choice for the quantitative and semi-quantitative detection of many types of proteins in complex mixtures. A combination of sensitivity, specificity and cost-effectiveness in terms of analytical performance are allied to a diverse array of assay formats suitable for laboratory and field use. In the present article, the problems of setting up immunoassays for novel food proteins, and the questions that need to be answered if a successful outcome is to be achieved, are discussed. Speculation on future developments in immunochemistry leads to the conclusion that antibody technology will play an important role in detection of novel proteins from genetically modified organisms. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：401 / 406

页数：6

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