Imaging the cAMP-dependent signal transduction pathway

被引：30

作者：

Zaccolo, M ^{[1
]}

Cesetti, T ^{[1
]}

Di Benedetto, G ^{[1
]}

Mongillo, M ^{[1
]}

Lissandron, V ^{[1
]}

Terrin, A ^{[1
]}

Zamparo, I ^{[1
]}

机构：

[1] Dulbecco Telethon Inst, Venetian Inst Mol Med, I-35100 Padua, Italy

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2005年 / 33卷

关键词：

biosensor; CAMP; cell imaging; fluorescence resonance energy transfer (FRET); protein kinase A; signal transduction;

D O I：

10.1042/BST0331323

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In recent years, the development of new technologies based on the green fluorescent protein and FRET (fluorescence resonance energy transfer) has introduced a new perspective in the study of CAMP signalling. Real-time imaging of fluorescent biosensors is making it possible to visualize CAMP dynamics directly as they happen in intact, living cells, providing important and original insights for our understanding of the spatiotemporal organization of the cAMP/PKA (protein kinase A) signalling pathway.

引用

页码：1323 / 1326

页数：4

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