Cloning and functional expression of a plant voltage-dependent chloride channel

被引：81

作者：

Lurin, C ^{[1
]}

Geelen, D ^{[1
]}

BarbierBrygoo, H ^{[1
]}

Guern, J ^{[1
]}

Maurel, C ^{[1
]}

机构：

[1] CNRS,INST SCI VEGETALES,F-91198 GIF SUR YVETTE,FRANCE

来源：

PLANT CELL | 1996年 / 8卷 / 04期

关键词：

D O I：

10.1105/tpc.8.4.701

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant cell membrane anion channels participate in basic physiological functions, such as cell volume regulation and signal transduction. However, nothing is known about their molecular structure. Using a polymerase chain reaction strategy, we have cloned a tobacco cDNA (CIC-Nt1) encoding a 780-amino acid protein with several putative transmembrane domains. CIC-Nt1 displays 24 to 32% amino acid identity with members of the animal voltage-dependent chloride channel (CIC) family, whose archetype is CIC-0 from the Torpedo marmorata electric organ. Injection of CIC-Nt1 complementary RNA into Xenopus oocytes elicited slowly activating inward currents upon membrane hyperpolarization more negative than -120 mV. These currents were carried mainly by anions, modulated by extracellular anions, and totally blocked by 10 mM extracellular calcium. The identification of CIC-Nt1 extends the CIC family to higher plants and provides a molecular probe for the study of voltage-dependent anion channels in plants.

引用

页码：701 / 711

页数：11

共 43 条

[1] SEQUENCE AND FUNCTIONAL EXPRESSION OF AN AMPHIBIAN WATER CHANNEL, FA-CHIP - A NEW MEMBER OF THE MIP FAMILY [J].