Gas-phase peptide/protein cationizing agent switching via ion/ion reactions

被引:40
作者
Newton, KA [1 ]
McLuckey, SA [1 ]
机构
[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
关键词
D O I
10.1021/ja036924e
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Polypeptide ions comprising different cationizing agents show distinct fragmentation behavior in the gas phase. Thus, it is desirable to be able to form ions with different cationizing agents such as protons and metal ions. Usually, metal-cationized peptide/protein ions are introduced to the mass spectrometer by electrospraying solutions containing a mixture of the peptide/protein of interest and a metal salt. A new technique for generating metal-containing polypeptide ions that involves gas-phase ion/ion reactions is described. In this strategy, solutions of metal-containing ions and solutions of proteins are each electrosprayed into separate ion sources. The approach allows for independent maximization of ion signal and selection of ions prior to gas-phase reactions. Selected ions are stored in a quadrupole ion trap where reactions of ions of opposite polarity form metal-cationized peptides and proteins in the gas phase by cation switching. This approach affords a high degree of flexibility in forming metal-containing peptide and protein ions via the ability to mass-select reactant ions. The ability to form a variety of peptide/protein ions with various cationizing reagents in the gas phase is attractive both for the study of intrinsic interactions of metal ions with polypeptides and for maximizing the structural information available from tandem mass spectrometry of peptides and proteins. Copyright © 2003 American Chemical Society.
引用
收藏
页码:12404 / 12405
页数:2
相关论文
共 11 条
[1]   A quadrupole ion trap mass spectrometer with three independent ion sources for the study of gas-phase ion/ion reactions [J].
Badman, Ethan R. ;
Chrisman, Paul A. ;
McLuckey, Scott A. .
Analytical Chemistry, 2002, 74 (24) :6237-6243
[2]   Influence of peptide composition, gas-phase basicity, and chemical modification on fragmentation efficiency: Evidence for the mobile proton model [J].
Dongre, AR ;
Jones, JL ;
Somogyi, A ;
Wysocki, VH .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (35) :8365-8374
[3]   INFLUENCES OF PEPTIDE SIDE-CHAINS ON THE METAL-ION BINDING-SITE IN METAL ION-CATIONIZED PEPTIDES - PARTICIPATION OF AROMATIC RINGS IN METAL CHELATION [J].
HU, PF ;
SORENSEN, C ;
GROSS, ML .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1995, 6 (11) :1079-1085
[4]   Complexes of silver(I) with peptides and proteins as produced in electrospray mass spectrometry [J].
Li, HB ;
Siu, KWM ;
Guevremont, R ;
LeBlanc, JCY .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1997, 8 (08) :781-792
[5]   Dissociation pathways of alkali-cationized peptides: Opportunities for C-terminal peptide sequencing [J].
Lin, T ;
Payne, AH ;
Glish, GL .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2001, 12 (05) :497-504
[6]  
McLuckey SA, 1998, MASS SPECTROM REV, V17, P369, DOI 10.1002/(SICI)1098-2787(1998)17:6<369::AID-MAS1>3.3.CO
[7]  
2-A
[8]   Gas-phase ion/ion interactions between peptides or proteins and iron ions in a quadrupole ion trap [J].
Payne, AH ;
Glish, GL .
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2001, 204 (1-3) :47-54
[9]   Formation and characterization of protein-protein complexes in vacuo [J].
Wells, JM ;
Chrisman, PA ;
McLuckey, SA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (24) :7238-7249
[10]   Dueling ESI: Instrumentation to study ion/ion reactions of electrospray-generated cations and anions [J].
Wells, JM ;
Chrisman, PA ;
McLuckey, SA .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2002, 13 (06) :614-622