The retromer complex influences Wnt secretion by recycling Wntless from endosomes to the trans-Golgi network

被引:244
作者
Belenkaya, Tatyana Y. [1 ]
Wu, Yihui [1 ]
Tang, Xiaofang [1 ,2 ]
Zhou, Bo [1 ,2 ]
Cheng, Longqiu [1 ]
Sharma, Yagya V. [3 ]
Yan, Dong [1 ,2 ]
Selva, Erica M. [3 ]
Lin, Xinhua [1 ,2 ]
机构
[1] Univ Cincinnati, Coll Med, Div Dev Biol, Cincinnati, OH 45229 USA
[2] Univ Cincinnati, Cincinnati Childrens Hosp Med Ctr, Grad Program Mol & Dev Biol, Cincinnati, OH 45229 USA
[3] Univ Delaware, Dept Biol Sci, Newark, DE 19716 USA
关键词
D O I
10.1016/j.devcel.2007.12.003
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Secreted Wnt proteins play essential roles in many biological processes during development and diseases. However, little is known about the mechanism(s) controlling Wnt secretion. Recent studies have identified Wntless (WIs) and the retromer complex as essential components involved in Wnt signaling. While WIs has been shown to be essential for Wnt secretion, the function(s) of the retromer complex in Wnt signaling is unknown. Here, we have examined a role of Vps35, an essential retromer subunit, in Wnt signaling in Drosophila and mammalian cells. We provide compelling evidence that the retromer complex is required for Wnt secretion. Importantly, Vps35 colocalizes in endosomes and interacts with WIs. WIs becomes unstable in the absence of retromer activity. Our findings link WIs and retromer functions in the same conserved Wnt secretion pathway. We propose that retromer influences Wnt secretion by recycling Wntless from endosomes to the trans-Golgi network (TGN).
引用
收藏
页码:120 / 131
页数:12
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