Expression of Mucor circinelloides gene for Δ6 desaturase results in the accumulation of high levels of γ-linolenic acid in transgenic tobacco

被引：7

作者：

Hao, Y. L. ^{[1
]}

Mei, X. H. ^{[1
]}

Zhao, F. ^{[1
]}

Yin, S. ^{[1
]}

Li, R. H. ^{[1
]}

Luo, Y. B. ^{[1
]}

机构：

[1] China Agr Univ, Dept Food Sci & Nutr Engn, Beijing 100083, Peoples R China

来源：

RUSSIAN JOURNAL OF PLANT PHYSIOLOGY | 2008年 / 55卷 / 01期

关键词：

Nicotiana tabacum; Mucor circinelloides; Delta; 6; desaturase; gamma-linolenic acid; GUS staining;

D O I：

10.1134/S1021443708010093

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

gamma-Linolenic acid (GLA; C18:3 Delta 6,9,12) is a nutritionally important fatty acid (FA) playing a vital role in biological structures and cellular functions, which is not produced in oil seed crops. Many oil seed plants, however, produce significant quantities of linoleic acid, a FA that could be converted into GLA by the enzyme Delta 6 desaturase, if it is present. As a first step to produce GLA in oil seed crops, we isolated a cDNA encoding the Delta 6-FA desaturase from filamentous fungus Mucor circinelloides M29. Expression of this gene in transgenic tobacco resulted in the accumulation of GLA to the levels of 23.1% of the total FA. The results suggested that it is feasible to introduce the M. circinelloides Delta 6 desaturase gene into conventional oil crop to produce a large amount of GLA for functional foods and pharmaceutical products.

引用

页码：77 / 81

页数：5

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