Modification of fatty acid composition in tomato (Lycopersicon esculentum) by expression of a borage Δ6-desaturase

被引:15
作者
Cook, D
Grierson, D
Jones, C
Wallace, A
West, G
Tucker, G
机构
[1] Univ Nottingham, Sch Biosci, Div Nutr Biochem, Loughborough LE12 5RD, Leics, England
[2] Univ Nottingham, Sch Biosci, Div Plant Sci, Loughborough LE12 5RD, Leics, England
关键词
tomato; fatty acids; desaturase; nutritional quality; genetic modification;
D O I
10.1385/MB:21:2:123
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The improvement of nutritional quality is one potential application for the genetic modification of plants. One possible target for such manipulation is the modification of fatty acid metabolism. In this work, expression of a borage Delta(6)-desaturase cDNA in tomato (Lycopersicon esculentum L.) has been shown to produce gamma-linolenic acid (GLA; 18:3 Delta(6,9,12)) and octadecatetraenoic acid (OTA; 18:4 Delta(6,9,12,15)) in transgenic leaf and fruit tissue. This genetic modification has also, unexpectedly, resulted in a reduction in the percentage of linoleic acid (LA 18:2 Delta(9,12)) and a concomitant increase in the percentage of alpha-linolenic acid (ALA; 18:3 Delta(9,12,15)) in fruit tissue. These changes in fatty acid composition are thought to be beneficial for human health.
引用
收藏
页码:123 / 128
页数:6
相关论文
共 14 条
[1]   Plants as 'chemical factories' for the production of polyunsaturated fatty acids [J].
Alonso, DL ;
Maroto, FG .
BIOTECHNOLOGY ADVANCES, 2000, 18 (06) :481-497
[2]  
Christie W., 1989, GAS CHROMATOGRAPHY L
[3]   DNA ANALYSES IN WHEAT BREEDING [J].
CLARKE, BC ;
MORAN, LB ;
APPELS, R .
GENOME, 1989, 32 (02) :334-339
[4]  
COUPLAND K, 1996, ISSFAL
[5]  
Department of Health, 1994, 46 DEP HLTH
[6]  
GALLIE DR, 1989, PLANT CELL, V1, P301, DOI 10.1105/tpc.1.3.301
[7]  
Gunstone F.D., 1994, LIPID HDB
[8]   The effect of chimeric transgene architecture on co-ordinated gene silencing [J].
Jones, CG ;
Scothern, GP ;
Lycett, GW ;
Tucker, GA .
PLANTA, 1998, 204 (04) :499-505
[9]   Plant desaturases: harvesting the fat of the land [J].
Napier, JA ;
Michaelson, LV ;
Stobart, AK .
CURRENT OPINION IN PLANT BIOLOGY, 1999, 2 (02) :123-127
[10]   Expression of a cyanobacterial Delta(6)-desaturase gene results in gamma-linolenic acid production in transgenic plants [J].
Reddy, AS ;
Thomas, TL .
NATURE BIOTECHNOLOGY, 1996, 14 (05) :639-642