Triacylglycerol analysis of potential margarine base stocks by high-performance liquid chromatography with atmospheric pressure chemical ionization mass spectrometry and flame ionization detection

被引:44
作者
Byrdwell, WC
Neff, WE
List, GR
机构
[1] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA
[2] Florida Atlantic Univ, Dept Biochem & Chem, Boca Raton, FL 33431 USA
关键词
atmospheric pressure chemical ionization mass spectrometry; flame ionization detector; margarine base stocks; triacylglycerol analysis; triglyceride; triacylglycerol;
D O I
10.1021/jf0008801
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Several margarine base stock candidates have previously been prepared for the purpose of finding better, more oxidatively stable food components: high-saturate vegetable oils, randomized vegetable oils, vegetable oil-hard stock blends, and interesterified vegetable oil-hard stock blends. Here are reported the triacylglycerol compositions of these products, determined using reverse-phase highperformance liquid chromatography (HPLC) coupled with a flame ionization detector or a quadrupole mass spectrometer with an atmospheric pressure chemical ionization source. Triacylglycerol percent composition results for samples of known composition (randomized and interesterified samples) exhibited less average error by HPLC coupled with a quadrupole mass spectrometer with an atmospheric pressure chemical ionization source, after application of response factors, than the results by HPLC coupled with a flame ionization detector. The fatty acid compositions calculated from the mass spectrometric data exhibited less average error than the fatty acid compositions resulting from the flame ionization detector data. The average error of the fatty acid compositions by the mass spectrometer was lowest for interesterified blend samples, next lowest for randomized samples, then followed by high-saturated fatty acid oils, normal oils, and blends. Analysis of the vegetable oil-hard stock blends by mass spectrometer required special treatment for calculation of response factors.
引用
收藏
页码:446 / 457
页数:12
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