A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Argot

被引：25

作者：

Deshmukh, Vishal P. ^{[1
]}

Thakare, Prashant V. ^{[1
]}

Chaudhari, Uddhav S. ^{[1
]}

Gawande, Prashant A. ^{[1
]}

机构：

[1] Sant Gadge Baba Amravati Univ, Dept Bot, Amritsar 444602, Punjab, India

来源：

ELECTRONIC JOURNAL OF BIOTECHNOLOGY | 2007年 / 10卷 / 03期

关键词：

Ayurveda; DNA extraction; medicinal plant; Terminalia species;

D O I：

10.2225/vol10-issue3-fulltext-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Current protocols for isolation of genomic DNA from Terminalia Arjuna have their own limitations due to the presence of high content of gummy polysaccharides and polyphenols. DNA isolated by these protocols is contaminated with a yellowish, sticky and viscous matrix. In our modified DNA isolation method polysaccharides and polyphenols are removed prior to the precipitation of the DNA. Then the genomic DNA was precipitated using isopropanol. This protocol yielded a high molecular weight DNA isolated from fresh as well as dry leaves of T. Arjuna, which was free from contamination and colour. Isolated DNA can be used for restriction digestion and PCR amplification. The main objective of the present protocol is to provide a simple method of isolation of DNA, using in house prepared reagents.

引用

页码：468 / 472

页数：5

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