Single-molecule RNA science

被引：129

作者：

Zhuang, XW ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA

[2] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 2005年 / 34卷

关键词：

single-molecule experiments; RNA folding; RNA catalysis; fluorescence resonance energy transfer; optical tweezers;

D O I：

10.1146/annurev.biophys.34.040204.144641

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of single-molecule detection and manipulation has allowed us to monitor the behavior of individual biological molecules and molecular complexes in real time. This approach significantly expands our capability to characterize complex dynamics of biological processes, allowing transient intermediate states and parallel kinetic pathways to be directly observed. Exploring this capability to elucidate complex dynamics, recent single-molecule experiments on RNA folding and catalysis have improved our understanding of the folding energy landscape of RNA and allowed us to better dissect complex RNA catalytic reactions, including translation by the ribosome.

引用

页码：399 / 414

页数：20

共 83 条

[1] OBSERVATION OF A SINGLE-BEAM GRADIENT FORCE OPTICAL TRAP FOR DIELECTRIC PARTICLES [J].

ASHKIN, A ;

DZIEDZIC, JM ;

BJORKHOLM, JE ;

CHU, S .

OPTICS LETTERS, 1986, 11 (05) :288-290

[2] Using single-particle tracking to study nuclear trafficking of viral genes [J].

Babcock, HP ;

Chen, C ;

Zhuang, XW .

BIOPHYSICAL JOURNAL, 2004, 87 (04) :2749-2758

[3] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[4] Exploration of the transition state for tertiary structure formation between an RNA helix and a large structured RNA [J].

Bartley, LE ;

Zhuang, XW ;

Das, R ;

Chu, S ;

Herschlag, D .

JOURNAL OF MOLECULAR BIOLOGY, 2003, 328 (05) :1011-1026

[5] tRNA selection and kinetic proofreading in translation [J].

Blanchard, SC ;

Gonzalez, RL ;

Kim, HD ;

Chu, S ;

Puglisi, JD .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (10) :1008-1014

[6] tRNA dynamics on the ribosome during translation [J].

Blanchard, SC ;

Kim, HD ;

Gonzalez, RL ;

Puglisi, JD ;

Chu, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (35) :12893-12898

[7] Single-molecule transition-state analysis of RNA folding [J].

Bokinsky, G ;

Rueda, D ;

Misra, VK ;

Rhodes, MM ;

Gordus, A ;

Babcock, HP ;

Walter, NG ;

Zhuang, XW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (16) :9302-9307

[8]

Buchmueller KL, 2000, NAT STRUCT BIOL, V7, P362

[9]

Burgstaller P, 2002, CURR OPIN DRUG DISC, V5, P690

[10] Ten years of tension: single-molecule DNA mechanics [J].

Bustamante, C ;

Bryant, Z ;

Smith, SB .

NATURE, 2003, 421 (6921) :423-427

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