Dose-dependent inhibition of gynecophoral canal protein gene expression in vitro in the schistosome (Schistosoma japonicum) by RNA interference

被引：32

作者：

Cheng, GF

Lin, JJ

Shi, Y

Jin, YX ^{[1
]}

Fu, ZQ

Jin, YM

Zhou, YC

Cai, YM

机构：

[1] Chinese Acad Agr Sci, Shanghai Inst Anim Parasitol, Key Lab Anim Parasitol, Minist Agr, Shanghai 200232, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2005年 / 37卷 / 06期

关键词：

D O I：

10.1111/j.1745-7270.2005.00058.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary for the pairing between the male and female worms is specifically expressed in the adult male worm. This protein is widely distributed in the adult female worm after pairing. Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence were employed to analyze the relationship between the RNAi effect and dsRNA dosage in the parasites. The results revealed that the inhibition of SjGCP expression by siRNA is dose-dependent. RT-PCR analysis showed that the SjGCP transcript level was reduced by 75% when 100 nM dsRNA was applied.

引用

页码：386 / 390

页数：5

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