Clarification of enhanced hydroxyl radical production in Fenton reaction with ATP/ADP based on luminol chemiluminescence

被引：6

作者：

Aoyagi, S

Yamazaki, M

Miyasaka, T

Sakai, K ^{[1
]}

机构：

[1] Waseda Univ, Dept Chem Engn, Tokyo 1698555, Japan

[2] Kawasaki Med Sch, Dept Physiol, Okayama 7010192, Japan

来源：

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | 2001年 / 34卷 / 07期

关键词：

ATP; ADP; luminol chemiluminescence; Fenton reaction; hydroxyl radical;

D O I：

10.1252/jcej.34.956

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Enhanced hydroxyl radical production in the Fenton reaction with ATP or ADP based on luminol chemiluminescence was clarified. Luminol chemiluminescence intensity increases with the concentration of ATP or ADP due to their influence on the Fenton reagents which produce hydroxyl radicals. Fenton reagents containing hydrogen peroxide and ferrous ion produce hydroxyl radicals which attack luminol and this emits light. The effect of ATP and ADP on the Fenton reaction is detected as luminol CL intensity with a photon counting system. This method allows measurement of ATP or ADP concentration ranging from 0 to 15 mM, which covers the physiological concentration range.

引用

页码：956 / 959

页数：4

共 14 条

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