Hydrogen peroxide assay by using enhanced chemiluminescence of the luminol-H2O2-horseradish peroxidase system: Comparative studies

p-Iodophenol, p-coumaric acid, phenol and aniline are enhancers of the luminol-H2O2-horseradish peroxidase chemiluminescence. These enhanced and unenhanced chemiluminescences were used to assay low concentrations of hydrogen peroxide. The curves of chemiluminescence intensity maxima against hydrogen peroxide concentration were fitted to third order polynomial regressions, which permit a very good approach to the experimental results. Double the sensitivity (RSD less than or equal to 4.3%) and a slightly lower detection limit (e.g. 0.08 mu M with p-coumaric acid of 0.18 mu M without enhancer) were obtained with enhanced chemiluminescence than with unenhanced chemiluminescence.