Genomic and biological analysis of Grapevine leafroll-associated virus 7 reveals a possible new genus within the family Closteroviridae

被引：64

作者：

Al Rwahnih, Maher ^{[1
]}

Dolja, Valerian V. ^{[2
]}

Daubert, Steve ^{[1
]}

Koonin, Eugene V. ^{[3
]}

Rowhani, Adib ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA

[2] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA

[3] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA

来源：

VIRUS RESEARCH | 2012年 / 163卷 / 01期

关键词：

Grapevine viruses; Leafroll disease; Closteroviridae family; GLRaV-7; Deep sequence analysis; LChV-1; CoV-1; MULTIPLE SEQUENCE ALIGNMENT; NUCLEOTIDE-SEQUENCE; DEGENERATE PRIMERS; GENETIC DIVERSITY; MOVEMENT PROTEIN; IDENTIFICATION; ORGANIZATION; MEMBER; RNAS;

D O I：

10.1016/j.virusres.2011.10.018

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Deep sequencing analysis of an asymptomatic grapevine revealed a virome containing five RNA viruses and a viroid. Of these, Grapevine leafroll-associated virus 7 (GLRaV-7), an unassigned closterovirus, was by far the most prominently represented sequence in the analysis. Graft-inoculation of the infection to another grape variety confirmed the lack of the leafroll disease symptoms, even though GLRaV-7 could be detected in the inoculated indicator plants. A 16,496 nucleotide-long genomic sequence of this virus was determined from the deep sequencing data. Its genome architecture and the sequences encoding its nine predicted proteins were compared with those of other closteroviruses. The comparison revealed that two other viruses, Little cherry virus-1 and Cordyline virus-1 formed a well supported phylogenetic cluster with GLRaV-7. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：302 / 309

页数：8

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