Development, optimization and validation of a fingerprint of Ginkgo biloba extracts by high-performance liquid chromatography

被引:91
作者
Ji, YB
Xu, QS
Hu, YZ
Heyden, YV
机构
[1] Free Univ Brussels, Inst Pharmaceut, Dept Pharmaceut & Biomed Anal, B-1090 Brussels, Belgium
[2] China Pharmaceut Univ, Nanjing 2100029, Peoples R China
关键词
HPLC fingerprint; method development; uniform design; Ginkgo biloba; herbal medicines; quality control;
D O I
10.1016/j.chroma.2005.01.035
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A four-step development, optimization and validation strategy for high-performance liquid chromatography (HPLC) fingerprints of Ginkgo biloba extract is described. A suitable chromatographic system was selected first. The following step was performing a screening design to select important parameters. After selecting some controllable parameters and their range to further optimize, gradient optimization with uniform design was done. At last, method validation including determination of injection precision, repeatability, and a sample stability test, was performed. Through this effective and integrated four-step method, a feasible and reliable HPLC fingerprint to identify and assess the Ginkgo biloba quality can easily be established using a linear gradient elution with acetonitrile/0.1% phosphoric acid (from 14/86 to 30/70, v/v, in 40 min) as mobile phase, a column temperature of 30degreesC and a detection wavelength of 350 nm. The strategy can also be applied for the development of fingerprints in the quality control of other herbal medicines. (C) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:97 / 104
页数:8
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