CCR9-positive lymphocytes and thymus-expressed chemokine distinguish small bowel from colonic Crohn's disease

被引:181
作者
Papadakis, KA
Prehn, J
Moreno, ST
Cheng, L
Kouroumalis, EA
Deem, R
Breaverman, T
Ponath, PD
Andrew, DP
Green, PHR
Hodge, MR
Binder, SW
Targan, SR
机构
[1] Univ Calif Los Angeles, Sch Med, Cedars Sinai Med Ctr, Ctr Inflammatory Bowel Dis, Los Angeles, CA 90048 USA
[2] Univ Calif Los Angeles, Sch Med, Cedars Sinai Med Ctr, Dept Med,Div Gastroenterol, Los Angeles, CA 90048 USA
[3] Cedars Sinai Med Ctr, Dept Pathol & Lab Med, Los Angeles, CA 90048 USA
[4] Univ Crete, Univ Hosp Heraklion, Dept Gastroenterol, Iraklion, Greece
[5] LeukoSite Inc, Cambridge, MA USA
[6] Columbia Univ Coll Phys & Surg, Dept Med, New York, NY USA
[7] Millennium Pharmaceut, Cambridge, MA USA
关键词
D O I
10.1053/gast.2001.27154
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background & Aims: Thymus-expressed chemokine (TECK) or CCL25) is selectively expressed in the small bowel (SIB), where lamina propria lymphocytes (LPL) and intraepithelial leukocyte expressing the cognate chemokine receptor CCR9 predominate. We characterize the role of TECK and CCR9-expresing lymphocytes in small intestinal Crohn's disease. Methods: CCR9 expression on lymphocytes from lamina propria, mesenteric lymph node, and peripheral blood was analyzed by flow cytometry and by Northern blotting for LPL. TECK expression was analyzed in inflamed SB and colon by reverse-transcription polymerase chain reaction and immunohistochemistry. Results : The fraction of CCR9(+) T cells in inflamed SB was significantly lower than in uninvolved SB mucosa. In contrast, in peripheral blood lymphocytes, CCR9+ lymphocytes were markedly elevated in patients with small bowel Crohn's or celiac disease, but not in patients with purely colonic Crohn's. Also, TECK expression is altered in inflamed small bowel, being intensely expressed in a patchy distribution in crypt epithelial cells in proximity to lymphocytic infiltrates. TECK is not expressed in either normal or inflamed colon. Conclusions: In SB immune-mediated diseases, there is repartitioning of CCR9+ lymphocytes between SB and blood and an altered pattern of TECK expression in SB Crohn's. The TECK/CCR9 ligand/receptor pair may play an important role in the pathogenesis of SB Crohn's disease.
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页码:246 / 254
页数:9
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