Sequential Immobilization of Enzymes in Microfluidic Channels for Cascade Reactions

被引：51

作者：

Fornera, Sara ^{[2
]}

Kuhn, Phillip ^{[1
]}

Lombardi, Dario ^{[1
]}

Schlueter, A. Dieter ^{[2
]}

Dittrich, Petra S. ^{[1
]}

Walde, Peter ^{[2
]}

机构：

[1] ETH, Dept Chem & Appl Biosci, CH-8093 Zurich, Switzerland

[2] ETH, Dept Mat, CH-8093 Zurich, Switzerland

来源：

CHEMPLUSCHEM | 2012年 / 77卷 / 02期

基金：

欧洲研究理事会; 瑞士国家科学基金会;

关键词：

beta-galactosidase; enzymes; glucose oxidase; horseradish peroxidase; lactose; microfluidic devices; MULTIENZYME SYSTEMS; CATALYSIS; POLYMER; KINETICS; DEVICES; DESIGN;

D O I：

10.1002/cplu.201100068

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Go with the flow: A flow-through microfluidic reactor system was developed that contains the three enzymes, β-galactosidase (β-Gal), glucose oxidase (GOD), and peroxidase (HRP), immobilized with a polycationic dendronized polymer in a predetermined sequence on a single chip for the conduction of an enzymatic cascade reaction. The desired enzyme localization within the microchannels of the chip could be achieved with a micromechanical valve system. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：98 / 101

页数：4

共 33 条

[1] Magnetolithographic Patterning of Inner Walls of a Tube: A New Dimension in Microfluidics and Sequential Microreactors [J].