Sequential Immobilization of Enzymes in Microfluidic Channels for Cascade Reactions

被引：51

作者：

Fornera, Sara ^{[2
]}

Kuhn, Phillip ^{[1
]}

Lombardi, Dario ^{[1
]}

Schlueter, A. Dieter ^{[2
]}

Dittrich, Petra S. ^{[1
]}

Walde, Peter ^{[2
]}

机构：

[1] ETH, Dept Chem & Appl Biosci, CH-8093 Zurich, Switzerland

[2] ETH, Dept Mat, CH-8093 Zurich, Switzerland

来源：

CHEMPLUSCHEM | 2012年 / 77卷 / 02期

基金：

欧洲研究理事会; 瑞士国家科学基金会;

关键词：

beta-galactosidase; enzymes; glucose oxidase; horseradish peroxidase; lactose; microfluidic devices; MULTIENZYME SYSTEMS; CATALYSIS; POLYMER; KINETICS; DEVICES; DESIGN;

D O I：

10.1002/cplu.201100068

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Go with the flow: A flow-through microfluidic reactor system was developed that contains the three enzymes, β-galactosidase (β-Gal), glucose oxidase (GOD), and peroxidase (HRP), immobilized with a polycationic dendronized polymer in a predetermined sequence on a single chip for the conduction of an enzymatic cascade reaction. The desired enzyme localization within the microchannels of the chip could be achieved with a micromechanical valve system. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

收藏

页码：98 / 101

页数：4

相关论文

共 33 条

[21] Multi-enzymatic synthesis [J].

Lopez-Gallego, Fernando ;

Schmidt-Dannert, Claudia .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2010, 14 (02) :174-183

[22] Silica-immobilized enzymes for multi-step synthesis in microfluidic devices [J].

Luckarift, Heather R. ;

Ku, Bosung S. ;

Dordick, Jonathan S. ;

Spain, Jim C. .

BIOTECHNOLOGY AND BIOENGINEERING, 2007, 98 (03) :701-705

[23] Design and characterization of immobilized enzymes in microfluidic systems [J].

Mao, HB ;

Yang, TL ;

Cremer, PS .

ANALYTICAL CHEMISTRY, 2002, 74 (02) :379-385

[24] Synthesis of enantiomerically pure (S)-mandelic acid using an oxynitrilase-nitrilase bienzymatic cascade:: a nitrilase surprisingly shows nitrile hydratase activity [J].

Mateo, C ;

Chmura, A ;

Rustler, S ;

van Rantwijk, F ;

Stolz, A ;

Sheldon, RA .

TETRAHEDRON-ASYMMETRY, 2006, 17 (03) :320-323

[25] Design and Characterization of a Prototype Enzyme Microreactor: Quantification of Immobilized Transketolase Kinetics [J].

Matosevic, S. ;

Lye, G. J. ;

Baganz, F. .

BIOTECHNOLOGY PROGRESS, 2010, 26 (01) :118-126

[26] The excited-states quenching of resazurin and resorufin by p-benzoquinones in polar solvents [J].

Montejano, HA ;

Gervaldo, M ;

Bertolotti, SG .

DYES AND PIGMENTS, 2005, 64 (02) :117-124

[27] Rapid one-pot synthesis of riboflavin isotopomers [J].

Römisch, W ;

Eisenreich, W ;

Richter, G ;

Bacher, A .

JOURNAL OF ORGANIC CHEMISTRY, 2002, 67 (25) :8890-8894

[28] Efficient mixing and reactions within microfluidic channels using microbead-supported catalysts [J].

Seong, GH ;

Crooks, RM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (45) :13360-13361

[29] Measurement of enzyme kinetics using a continuous-flow microfluidic system [J].

Seong, GH ;

Heo, J ;

Crooks, RM .

ANALYTICAL CHEMISTRY, 2003, 75 (13) :3161-3167

[30] E factors, green chemistry and catalysis: an odyssey [J].

Sheldon, Roger A. .

CHEMICAL COMMUNICATIONS, 2008, (29) :3352-3365

← 1 2 3 4 →