HBV X protein targets HIV tat-binding protein 1

被引:27
作者
Barak, O
Aronheim, A
Shaul, Y [1 ]
机构
[1] Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel
[2] Technion Israel Inst Technol, Rappaport Fac Med, Dept Mol Genet, IL-31096 Haifa, Israel
关键词
HBV pX; transcription; 19S; AAA protein family; Sos recruitment system (SRS); HCC;
D O I
10.1006/viro.2001.0883
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The HBV X protein (HBx) is implicated in infection and development of hepatocellular carcinoma. HBx has a pleiotropic effect on cells, suggesting multiple targets in the virus-host cell interaction. We employed the cytoplasmic-based two-hybrid screen and identified the HIV Tat-binding protein 1 (Tbp1) as a novel HBx interacting protein. Tbp1 interacts in vivo with HBx both in yeast and in animal cells. This interaction maps to the functionally important ATP-binding motif of Tbp1. Furthermore, HBx and Tbp1 interaction is functionally significant and regulates HBV transcription. Tbpl homologues, such as Sug1, are known members of the proteasome 19S regulatory cap particle and have also been implicated in transcription coactivation. Remarkably, Tbp1 and Sug1 interact with multiple viral effector proteins including HIV Tat, SV40 large T antigen, and adenovirus E1A, establishing these proteins as important targets of the viral oncogenes. (C) 2001 Academic Press.
引用
收藏
页码:110 / 120
页数:11
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