Smad5 is required for mouse primordial germ cell development

被引：153

作者：

Chang, H

Matzuk, MM

机构：

[1] Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA

[2] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA

[3] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA

[4] Baylor Coll Med, Program Dev Biol, Houston, TX 77030 USA

来源：

MECHANISMS OF DEVELOPMENT | 2001年 / 104卷 / 1-2期

关键词：

TGF-beta; SMAD; alkaline phosphatase; BMP; Fast Red staining; Oct4; embryogenesis;

D O I：

10.1016/S0925-4773(01)00367-7

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Smad5, together with Smad1 and Smad8, have been implicated as downstream signal mediators for several bone morphogenetic proteins (BMPs). Recent studies have shown that primordial germ cells (PGCs) are absent or greatly reduced in Bmp4 or Bmp8b mutant mice. To define the role of Smad5 in PGC development, we examined PGC number in Smad5 mutant mice by Oct4 whole-mount in situ hybridization and alkaline phosphatase staining. We found ectopic PGC-like cells in the amnion of some Smad5 mutant mice, however, the total number of PGCs was greatly reduced or completely absent in Smad5 mutant embryos, similar to Bmp4 or Bmp8b mutant embryos. Therefore, Smad5 is an important factor involved in PGC generation and localization. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：61 / 67

页数：7