Role of TGF-β1-independent changes in protein neosynthesis, p380αMAPK and cdc42 in hydrogen peroxide-induced senescence-like morphogenesis

被引：18

作者：

Chretien, Aline ^{[1
]}

Dierick, Jean-Francois ^{[2
]}

Delaive, Edouard ^{[1
]}

Larsen, Martin Rossel ^{[3
]}

Dieu, Marc ^{[1
]}

Raes, Martine ^{[1
]}

Deroanne, Christophe F. ^{[4
]}

Roepstorff, Peter ^{[3
]}

Toussaint, Olivier ^{[1
]}

机构：

[1] Univ Namur, Res Unit Cellular Biol, B-5000 Namur, Belgium

[2] Univ Brussels, Inst Mol Biol & Med, Gosselies, Belgium

[3] Univ So Denmark, Dept Biochem & Mol Biol, Prot Res Grp, Odense M, Denmark

[4] Univ Liege, CBIG GIGA Res Ctr, Lab Connect Tissues Biol, Sart Tilman Par Liege, Belgium

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 2008年 / 44卷 / 09期

关键词：

H2O2; neosynthesis; proteomics; siRNA; senescence; cdc42; p38(MAPK); free radicals;

D O I：

10.1016/j.freeradbiomed.2008.01.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of TGF-beta 1 in hydrogen peroxide-induced senescence-like morphogenesis has been described. The aim of this work was to investigate whether TGF-beta 1-independent changes in protein synthesis are involved in this morphogenesis and to study possible mechanisms occurring earlier than TGF-beta 1 overexpression. Among the multiple TGF-beta 1-independent changes in protein neosynthesis, followed or not by posttranslational modifications, identified by proteomic analysis herein, those of ezrin, L-caldesmon, and HSP27 were particularly studied. Rho-GTPase cdc42 was shown to be responsible for p38(MAPK) activation, in turn triggering phosphorylation of L-caldesmon and HSP27. Cdc42 was also shown to be mainly responsible for the increase in TGF beta 1 mRNA level observed at 24 h after treatment with H2O2 and onward. This study further clarified the mechanisms of senescence-like morphogenesis in addition to the previously demonstrated role of TGF-beta 1 signaling pathways. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：1732 / 1751

页数：20

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