cDNA cloning of a mouse pituitary adenylate cyclase-activating polypeptide receptor

被引：38

作者：

Hashimoto, H ^{[1
]}

Yamamoto, K ^{[1
]}

Hagihara, N ^{[1
]}

Ogawa, N ^{[1
]}

Nishino, A ^{[1
]}

Aino, H ^{[1
]}

Nogi, H ^{[1
]}

Imanishi, K ^{[1
]}

Matsuda, T ^{[1
]}

Baba, A ^{[1
]}

机构：

[1] OSAKA UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL,SUITA,OSAKA 565,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1996年 / 1281卷 / 02期

关键词：

pituitary adenylate cyclase-activating polypeptide receptor; PACAP receptor; cDNA cloning; vasoactive intestinal polypeptide; (mouse);

D O I：

10.1016/0005-2736(96)00056-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone for mouse pituitary adenylate cyclase-activating polypeptide (PACAP) receptor (PACAP-R) was obtained from the brain using reverse transcription-polymerase chain reaction (RT-PCR). The recombinant PACAP receptor expressed in COS cells bound PACAP with about 1000-times higher affinity than vasoactive intestinal polypeptide (VIP), and PACAP stimulated adenylate cyclase through the cloned PACAP receptor. The mouse PACAP receptor consists of 496 amino acids, contains seven transmembrane segments and has 98.4%, 93.0%, and 92.5% identity with the rat, bovine, and human PACAP-R, respectively.

引用

收藏

页码：129 / 133

页数：5

相关论文

共 21 条

[1] STRUCTURE OF THE GENE ENCODING THE MOUSE PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE RECEPTOR [J].

AINO, H ;

HASHIMOTO, H ;

OGAWA, N ;

NISHINO, A ;

YAMAMOTO, K ;

NOGI, H ;

NAGATA, S ;

BABA, A .

GENE, 1995, 164 (02) :301-304

[2]

ARIMURA A, 1992, REGUL PEPTIDES, V37, P287

[3]

CHIK CL, 1995, J NEUROCHEM, V64, P2111

[4] 38-AMINO ACID FORM OF PITUITARY ADENYLATE-CYCLASE ACTIVATING PEPTIDE INDUCES PROCESS OUTGROWTH IN HUMAN NEUROBLASTOMA-CELLS [J].

DEUTSCH, PJ ;

SCHADLOW, VC ;

BARZILAI, N .

JOURNAL OF NEUROSCIENCE RESEARCH, 1993, 35 (03) :312-320

[5]

DEUTSCH PJ, 1992, J BIOL CHEM, V267, P5108

[6] MOLECULAR-CLONING AND TISSUE DISTRIBUTION OF A RECEPTOR FOR PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE [J].

HASHIMOTO, H ;

ISHIHARA, T ;

SHIGEMOTO, R ;

MORI, K ;

NAGATA, S .

NEURON, 1993, 11 (02) :333-342

[7] MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF RAT CDNAS ENCODING THE RECEPTOR FOR PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) [J].

HOSOYA, M ;

ONDA, H ;

OGI, K ;

MASUDA, Y ;

MIYAMOTO, Y ;

OHTAKI, T ;

OKAZAKI, H ;

ARIMURA, A ;

FUJINO, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 194 (01) :133-143

[8] CLONING AND FUNCTIONAL-CHARACTERIZATION OF A 3RD PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE RECEPTOR SUBTYPE EXPRESSED IN INSULIN-SECRETING CELLS [J].

INAGAKI, N ;

YOSHIDA, H ;

MIZUTA, M ;

MIZUNO, N ;

FUJII, Y ;

GONOI, T ;

MIYAZAKI, J ;

SEINO, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (07) :2679-2683

[9] FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF A NOVEL RECEPTOR FOR VASOACTIVE INTESTINAL POLYPEPTIDE [J].

ISHIHARA, T ;

SHIGEMOTO, R ;

MORI, K ;

TAKAHASHI, K ;

NAGATA, S .

NEURON, 1992, 8 (04) :811-819

[10] CA2+-DEPENDENT STIMULATORY EFFECT OF PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE ON CATECHOLAMINE SECRETION FROM CULTURED PORCINE ADRENAL-MEDULLARY CHROMAFFIN CELLS [J].

ISOBE, K ;

NAKAI, T ;

TAKUWA, Y .

ENDOCRINOLOGY, 1993, 132 (04) :1757-1765