Exploiting sequence space:: shuffling in vivo formed complementarity determining regions into a master framework

被引：59

作者：

Jirholt, P

Ohlin, M

Borrebaeck, CAK

Söderlind, E

机构：

[1] Univ Lund, Dept Immunotechnol, S-22007 Lund, Sweden

[2] BioInvent Therapeut AB, Lund, Sweden

来源：

GENE | 1998年 / 215卷 / 02期

关键词：

immunoglobulin germline gene; synthetic gene library; primer design; gene assembly; CDR shuffling;

D O I：

10.1016/S0378-1119(98)00317-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：471 / 476

页数：6

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