Fluorescence Imaging of Influenza H1N1 mRNA in Living Infected Cells Using Single-Chromophore FIT-PNA

被引：96

作者：

Kummer, Susann ^{[1
]}

Knoll, Andrea ^{[2
]}

Socher, Elke ^{[2
]}

Bethge, Lucas ^{[2
]}

Herrmann, Andreas ^{[1
]}

Seitz, Oliver ^{[2
]}

机构：

[1] Humboldt Univ, Dept Biol, Invalidenstr 42, D-10115 Berlin, Germany

[2] Humboldt Univ, Dept Chem, D-12489 Berlin, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 08期

关键词：

confocal microscopy; hybridization probes; molecular beacons; RNA; viruses; PEPTIDE NUCLEIC-ACID; MOLECULAR BEACONS; THIAZOLE ORANGE; PROBES; DNA; BASE; HYBRIDIZATION; REDUCTION; TRANSPORT; VIRUS;

D O I：

10.1002/anie.201005902

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A message from the virus was detected by a peptide nucleic acid probe that contains thiazole orange as a fluorescent base surrogate . The high specificity and biostability of the probes lead to significant improvements to the signal-to-background ratio in imaging the mRNA from the influenza H1N1 virus in living infected cells. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1931 / 1934

页数：4

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