Identification and characterization of microsatellite DNA markers developed in ide Leuciscus idus and Siberian roach Rutilus rutilus

被引:24
作者
Barinova, A
Yadrenkina, E
Nakajima, M
Taniguchi, N [1 ]
机构
[1] Tohoku Univ, Grad Sch Agr Sci, Lab Appl Populat Genet Informat, Sendai, Miyagi 9818555, Japan
[2] Russian Acad Sci, Inst Anim Systemat & Ecol, Siberian Branch, Novosibirsk 630091, Russia
来源
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 01期
关键词
bream; Cyprinidae; dace; ide; microsatellite DNA; Siberian roach;
D O I
10.1046/j.1471-8286.2003.00577.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The first five microsatellite markers for the ide, Leuciscus idus, and four microsatellite markers for the Siberian roach, Rutilus rutilus, were designed. Cross-amplification of ide markers was examined in Siberian roach and vice versa. The number of alleles per locus ranged from three to 13 in ide and from two to eight in roach. The expected heterozygosity ranged from 0.313 to 0.909 in ide and from 0.119 to 0.775 in roach. These markers could be used to evaluate the genetic population structure of these species and other fish from the Cyprinidae family.
引用
收藏
页码:86 / 88
页数:3
相关论文
共 6 条
[1]   Introduced and translocated fish species in the inland waters of Greece [J].
Economidis, PS ;
Dimitriou, E ;
Pagoni, R ;
Michaloudi, E ;
Natsis, L .
FISHERIES MANAGEMENT AND ECOLOGY, 2000, 7 (03) :239-250
[2]  
Hall S.R., 2000, Aquatic Ecosystem Health & Management, V3, P105, DOI 10.1016/S1463-4988(99)00070-6
[3]   GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].
RAYMOND, M ;
ROUSSET, F .
JOURNAL OF HEREDITY, 1995, 86 (03) :248-249
[4]   A COMPUTER-PROGRAM FOR CHOOSING OPTIMAL OLIGONUCLEOTIDES FOR FILTER HYBRIDIZATION, SEQUENCING AND INVITRO AMPLIFICATION OF DNA [J].
RYCHLIK, W ;
RHOADS, RE .
NUCLEIC ACIDS RESEARCH, 1989, 17 (21) :8543-8551
[5]  
Sambrook J, 1989, MOL CLONING LAB MANU
[6]   Isolation and characterization of microsatellite loci from red sea bream Pagrus major and detection in closely related species [J].
Takagi, M ;
Taniguchi, N ;
Cook, D ;
Doyle, RW .
FISHERIES SCIENCE, 1997, 63 (02) :199-204