Fabrication of a silica coating on magnetic γ-Fe2O3 nanoparticles by an immobilized enzyme

被引：60

作者：

Shukoor, Mohammed Ibrahim ^{[1
]}

Natalio, Filipe ^{[2
]}

Therese, Helen Annal ^{[1
]}

Tahir, Muhammad Nawaz ^{[1
]}

Ksenofontov, Vadim ^{[1
]}

Panthoefer, Martin ^{[1
]}

Eberhardt, Marc ^{[3
]}

Theato, Patrick ^{[3
]}

Schroeder, Heinz Christoph ^{[2
]}

Mueller, Werner E. G. ^{[2
]}

Tremel, Wolfgang ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Inst Anorgan Chem & Analyt Chem, D-55099 Mainz, Germany

[2] Johannes Gutenberg Univ Mainz, Inst Physiol Chem, Angew Mol Biol Abt, D-55099 Mainz, Germany

[3] Johannes Gutenberg Univ Mainz, Inst Organ Chem, D-55099 Mainz, Germany

来源：

CHEMISTRY OF MATERIALS | 2008年 / 20卷 / 11期

关键词：

D O I：

10.1021/cm7029954

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Silicatein, a hydrolytic protein encountered in marine sponges, was immobilized on maghemite (gamma-Fe2O3) nanoparticles that were surface functionalized with a reactive mulfunctional polymer. This polymer carries an anchor group based on dopamine which is capable of binding to the gamma-Fe2O3 Surface and a reactive functional group which allows binding of various biomolecules onto inorganic nanoparticles. This functional nitrilotriacetic acid (NTA) group allows immobilization of His-tagged silicatein on the surface of the gamma-Fe2O3 nanoparticles. The surface-bound protein retains its native hydrolytic activity to catalyze formation of silica through copolymerization of alkoxysilanes Si(OR)(4). Functionalization of the magnetic nanoparticles and the architecture of the SiO2-coated gamma-Fe2O3 nanoparticles was confirmed by TEM studies as well as by FT-IR and optical microscopy.

引用

页码：3567 / 3573

页数：7

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