Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae

被引：43

作者：

Roumanie, O ^{[1
]}

Weinachter, C ^{[1
]}

Larrieu, I ^{[1
]}

Crouzet, M ^{[1
]}

Doignon, F ^{[1
]}

机构：

[1] CNRS, UMR 5095, Inst Biochim & Genet Cellulaires, Lab Biol Mol & Sequencage, F-33076 Bordeaux, France

来源：

FEBS LETTERS | 2001年 / 506卷 / 02期

关键词：

Rho protein; GTPase activating protein; BAG7; LRG1; RGD2; Saccharomyces cerevisiae;

D O I：

10.1016/S0014-5793(01)02906-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rho proteins are down-regulated in vivo by specific GTPase activating proteins (RhoGAP). We have functionally studied three Saccharomyces cerevisiae putative RhoGAP. By first identifying Rho partners with a systematic two-hybrid approach and then using an in vitro assay, we have demonstrated that the Bag7 protein stimulated the GTPase activity of the Rho1 protein, Lrg1p acted on the Cdc42 and Rho2 GTPases and we showed that Rgd2p has a GAP activity on both Cdc42p and Rho5p. In addition, we brought the first evidence for the existence of a sixth functional Rho in yeast, the Cdc42/Rac-like GTPase Rho5. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：149 / 156

页数：8

共 40 条

[31] Small GTP-binding proteins [J].

Takai, Y ;

Sasaki, T ;

Matozaki, T .

PHYSIOLOGICAL REVIEWS, 2001, 81 (01) :153-208

[32] CLUSTAL-W - IMPROVING THE SENSITIVITY OF PROGRESSIVE MULTIPLE SEQUENCE ALIGNMENT THROUGH SEQUENCE WEIGHTING, POSITION-SPECIFIC GAP PENALTIES AND WEIGHT MATRIX CHOICE [J].

THOMPSON, JD ;

HIGGINS, DG ;

GIBSON, TJ .

NUCLEIC ACIDS RESEARCH, 1994, 22 (22) :4673-4680

[33] Cdc42-interacting protein 4 mediates binding of the Wiskott-Aldrich syndrome protein to microtubules [J].

Tian, L ;

Nelson, DL ;

Stewart, DM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (11) :7854-7861

[34]

VAN AL, 1997, GENE DEV, V11, P2295

[35] THE RHO-GAP ENCODED BY BEM2 REGULATES CYTOSKELETAL STRUCTURE IN BUDDING YEAST [J].

WANG, TT ;

BRETSCHER, A .

MOLECULAR BIOLOGY OF THE CELL, 1995, 6 (08) :1011-1024

[36] Yeast Lrg1p acts as a specialized RhoGAP regulating 1,3-β-glucan synthesis [J].

Watanabe, D ;

Abe, M ;

Ohya, Y .

YEAST, 2001, 18 (10) :943-951

[37] A CONTINUOUS SPECTROPHOTOMETRIC ASSAY FOR INORGANIC-PHOSPHATE AND FOR MEASURING PHOSPHATE RELEASE KINETICS IN BIOLOGICAL-SYSTEMS [J].

WEBB, MR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :4884-4887

[38] INTERACTION OF GTPASE-ACTIVATING PROTEIN WITH P21(RAS), MEASURED USING A CONTINUOUS ASSAY FOR INORGANIC-PHOSPHATE RELEASE [J].

WEBB, MR ;

HUNTER, JL .

BIOCHEMICAL JOURNAL, 1992, 287 :555-559

[39]

ZHENG Y, 1995, METHOD ENZYMOL, V256, P77

[40]

ZHENG Y, 1994, J BIOL CHEM, V269, P2369

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