Detection of the Bacillus anthracis gyrA gene by using a minor groove binder probe

被引：100

作者：

Hurtle, W

Bode, E

Kulesh, DA

Kaplan, RS

Garrison, J

Bridge, D

House, M

Frye, MS

Loveless, B

Norwood, D

机构：

[1] USA, Med Res Inst Infect Dis, Diagnost Syst Div, Ft Detrick, MD 21702 USA

[2] Clin Res Management, N Royalton, OH 44133 USA

[3] Battelle Mem Inst, Columbus, OH USA

[4] Geo Ctr, Newton, MA USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 01期

关键词：

D O I：

10.1128/JCM.42.1.179-185.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis, Bacillus cereus, and Bacillus thuringiensis. We evaluated the bacterial gyrA gene as a potential chromosomal marker for B. anthracis. A real-time PCR assay was developed for the detection of B. anthracis. After analysis of the unique nucleotide sequence of the B. anthracis gyrA gene, a fluorescent 3' minor groove binding probe was tested with 171 organisms from 29 genera of bacteria, including 102 Bacillus strains. The assay was found to be specific for all 43 strains of B. anthracis tested. In addition, a test panel of 105 samples was analyzed to evaluate the potential diagnostic capability of the assay. The assay showed 100% specificity, demonstrating the usefulness of the gyrA gene as a specific chromosomal marker for B. anthracis.

引用

页码：179 / 185

页数：7

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