A link between RNA metabolism and silencing affecting Arabidopsis development

被引:314
作者
Gregory, Brian D. [1 ,2 ]
O'Malley, Ronan C. [1 ,2 ]
Lister, Ryan [1 ,2 ]
Urich, Mark A. [1 ]
Tonti-Filippini, Julian [3 ]
Chen, Huaming [2 ]
Millar, A. Harvey [3 ]
Ecker, Joseph R. [1 ,2 ]
机构
[1] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA
[2] Salk Inst Biol Studies, Genome Anal Lab, La Jolla, CA 92037 USA
[3] Univ Western Australia, ARC Ctr Excellence Plant Energy Biol, Crawley, WA 6009, Australia
基金
美国国家科学基金会; 澳大利亚研究理事会;
关键词
D O I
10.1016/j.devcel.2008.04.005
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are abundant endogenous small RNAs (smRNAs) that control transcript expression through posttranscriptional gene silencing. Here, we show that concomitant loss of XRN4/EIN5, a 5-3'exoribonuclease, and ABH1/CBP80, a subunit of the mRNA cap binding complex, results in Arabidopsis plants manifesting myriad developmental defects. We find that ABH1/CBP80 is necessary to obtain proper mature miRNA levels, which suggests this protein affects the miRNA-mediated RNA silencing pathway. Additionally, we show that XRN4/ElN5 affects the levels of a smRNA class that is processed from both sense and antisense strands of similar to 130 endogenous transcripts that apparently are converted to double-stranded RNA (dsRNA) and subsequently processed. We find that the parent transcripts of these smRNAs accumulate in an uncapped form upon loss of XRN4/EIN5, which suggests that uncapped endogenous transcripts can become smRNA biogenesis substrates. Overall, our results reveal unexpected connections between RNA metabolism and silencing pathways.
引用
收藏
页码:854 / 866
页数:13
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