Longevity of lobsters is linked to ubiquitous telomerase expression

被引：85

作者：

Klapper, W ^{[1
]}

Kühne, K ^{[1
]}

Singh, KK ^{[1
]}

Heidorn, K ^{[1
]}

Parwaresch, R ^{[1
]}

Krupp, G ^{[1
]}

机构：

[1] Univ Kiel, Ctr Pathol & Appl Canc Res, Inst Hematopathol, D-24105 Kiel, Germany

来源：

FEBS LETTERS | 1998年 / 439卷 / 1-2期

关键词：

indeterminate growth; cell proliferation; senescence; telomerase assay; telomeric repeat sequence; Homarus americanus;

D O I：

10.1016/S0014-5793(98)01357-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammals have high growth rates in embryonic and juvenile phases and no growth in adult and senescent phases. We analyzed telomerase activity in a fundamentally different animal which grows indeterminately. Lobsters (Homarus americanus) grow throughout their life and the occurrence of senescence is slow. A modified TRAP assay was developed and the lobster telomeric repeat sequence TTAGG was determined, We detected telomerase activities which were dependent on RNA and protein components, required dGTP, dATP and dTTP, but not dCTP. Telomerase products with a five nucleotide periodicity were generated. High telomerase activities were detected in all lobster organs. We conclude that telomerase activation is a conserved mechanism for maintaining long-term cell proliferation capacity and preventing senescence, not only in cellular models or embryonic life stages but also in adult multicellular organisms. (C) 1998 Federation of European Biochemical Societies.

引用

页码：143 / 146

页数：4

共 27 条

[1] Telomerase activity: A biomarker of cell proliferation, not malignant transformation [J].

Belair, CD ;

Yeager, TR ;

Lopez, PM ;

Reznikoff, CA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (25) :13677-13682

[2] STRUCTURE AND FUNCTION OF TELOMERES [J].

BLACKBURN, EH .

NATURE, 1991, 350 (6319) :569-573

[3] TELOMERE ELONGATION IN IMMORTAL HUMAN-CELLS WITHOUT DETECTABLE TELOMERASE ACTIVITY [J].

BRYAN, TM ;

ENGLEZOU, A ;

GUPTA, J ;

BACCHETTI, S ;

REDDEL, RR .

EMBO JOURNAL, 1995, 14 (17) :4240-4248

[4] Characterization and developmental patterns of telomerase expression in plants [J].

Fitzgerald, MS ;

McKnight, TD ;

Shippen, DE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14422-14427

[5]

FOWLER RF, 1985, J BIOL CHEM, V260, P1296

[6]

Govind C.K., 1995, P291, DOI 10.1016/B978-012247570-2/50034-7

[7] Telomerase activity in the regenerative basal layer of the epidermis in human skin and in immortal and carcinoma-derived skin keratinocytes [J].

HarleBachor, C ;

Boukamp, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (13) :6476-6481

[8] TELOMERE LOSS - MITOTIC CLOCK OR GENETIC TIME BOMB [J].

HARLEY, CB .

MUTATION RESEARCH, 1991, 256 (2-6) :271-282

[9] ANALYSIS OF THE GENE ENCODING THE RNA SUBUNIT OF RIBONUCLEASE-P FROM THERMUS-THERMOPHILUS HB8 [J].

HARTMANN, RK ;

ERDMANN, VA .

NUCLEIC ACIDS RESEARCH, 1991, 19 (21) :5957-5964

[10]

Heller K, 1996, MOL GEN GENET, V252, P342, DOI 10.1007/BF02173780

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