A Neutralizing Antibody Selected from Plasma Cells That Binds to Group 1 and Group 2 Influenza A Hemagglutinins

被引：952

作者：

Corti, Davide ^{[2
,3
]}

Voss, Jarrod ^{[1
]}

Gamblin, Steven J. ^{[1
]}

Codoni, Giosiana ^{[3
]}

Macagno, Annalisa ^{[3
]}

Jarrossay, David ^{[3
]}

Vachieri, Sebastien G. ^{[1
]}

Pinna, Debora ^{[3
]}

Minola, Andrea ^{[3
]}

Vanzetta, Fabrizia ^{[2
]}

Silacci, Chiara ^{[3
]}

Fernandez-Rodriguez, Blanca M. ^{[3
]}

Agatic, Gloria ^{[2
]}

Bianchi, Siro ^{[2
]}

Giacchetto-Sasselli, Isabella ^{[3
]}

Calder, Lesley ^{[1
]}

Sallusto, Federica ^{[3
]}

Collins, Patrick ^{[1
]}

Haire, Lesley F. ^{[1
]}

Temperton, Nigel ^{[4
]}

Langedijk, Johannes P. M. ^{[5
]}

Skehel, John J. ^{[1
]}

Lanzavecchia, Antonio ^{[3
,6
]}

机构：

[1] Natl Inst Med Res, MRC, London NW7 1AA, England

[2] Humabs BioMed SA, CH-6500 Bellinzona, Switzerland

[3] Inst Res Biomed, CH-6500 Bellinzona, Switzerland

[4] Univ Kent, Sch Pharm, Viral Pseudotype Unit, Chatham ME4 4TB, Kent, England

[5] Pepscan Therapeut BV, NL-8243 RC Lelystad, Netherlands

[6] ETH, Inst Microbiol, CH-8032 Zurich, Switzerland

来源：

SCIENCE | 2011年 / 333卷 / 6044期

基金：

英国医学研究理事会; 英国惠康基金; 瑞士国家科学基金会; 欧洲研究理事会;

关键词：

MEMBRANE-FUSION; RECEPTOR-BINDING; MONOCLONAL-ANTIBODIES; VIRUS HEMAGGLUTININ; B-CELLS; COMPLEX; SEQUENCES; SUBTYPES; EPITOPE; SITE;

D O I：

10.1126/science.1205669

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The isolation of broadly neutralizing antibodies against influenza A viruses has been a long-sought goal for therapeutic approaches and vaccine design. Using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (HA) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza A viruses. Passive transfer of this antibody conferred protection to mice and ferrets. Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain. This antibody may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency.

引用

页码：850 / 856

页数：7

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